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BellBrook
IDH1 (human) (rec.) (His) (active) (5µg)
CHF 0.00
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Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Isocitrate Dehydrogenase 1; Isocitrate Dehydrogenase [NADP] Cytoplasmic, wild-type; Cytosolic NADP-isocitrate dehydrogenase; IDPc |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
Full-length active human IDH1 enzyme (aa1-414) with an N-terminal 6xHis tag. |
| Crossreactivity | Human |
| Application |
Drug Discovery |
| Biological Activity |
The enzyme has been thoroughly validated with the Hydriscreener NADPH Assay Kit. |
| MW | 47.5kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | O75874 |
| Formulation | Liquid in 50mM Tris-HCl, 100mM NaCl, 1mM TCEP, 20% Glycerol, (pH 7.5). |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer Uniprot O75874: IDH1 |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -80°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Scientific Background Information
Product Description
IDH1 is a cytosolic and peroxisomal NADP+-dependent enzyme that catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate (2-oxoglutarate), generating NADPH and CO₂. Through the production of NADPH, IDH1 contributes to cellular redox homeostasis, lipid metabolism and protection against oxidative stress. The α-ketoglutarate generated by IDH1 also serves as an important metabolic intermediate and cofactor for multiple α-ketoglutarate-dependent enzymes. Mutations in IDH1, particularly at residue R132, occur in several cancers and result in a neomorphic enzymatic activity that converts α-ketoglutarate to the oncometabolite D-2-hydroxyglutarate (2-HG). IDH1 is therefore an important target in cancer metabolism and drug discovery. Active recombinant IDH1 is suitable for studies of enzyme kinetics, NADPH production, metabolic regulation and for screening and characterization of IDH1 inhibitors and modulators.