Transcreener® cGAMP cGAS FP Assay

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BBL-3024-1K1000 assaysCHF 910.00
BBL-3024-10K10000 assaysCHF 5'261.00
BBL-3024-100K10 x 10000 assaysINQ
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Product Details
Product Type Kit
Properties
Application Set Compound Screening
Crossreactivity All
Quantity

1000 assays/384wells (BBL-3024-1K)

10000 assays/384wells (BBL-3024-10K)

Note: The exact number of assays depends on enzyme reaction conditions.

Sensitivity Excellent data quality (Z’ ≥ 0.7) and signal (≥85 mP polarization shift) at cGAMP ranges between 0.5 μM and 5 μM.
Detection Type Fluorescent polarization
Kit Contains

cGAMP Antibody, cGAMP ATTO 633 Tracer, Stop & Detect Buffer B, 10X, ATP, GTP, cGAMP, Interferon Stimulatory DNA

Note:  The plates are not included, please see manual for material not provided but necessary for assay.

Other Product Data

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Our product description may differ slightly from the original manufacturers product datasheet.


Assay Features

Easy-to-Use (mix-and-read method). Simple homogenous biochemical assay. Direct detection of unlabeled cGAMPSafe, non-radioactive method. HTS Compatible with 96, 384, and 1536-well plates. Robust Assay Z’ > 0.7 under initial velocity conditions.

Declaration Manufactured by BellBrook Labs.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Documents
Manual Download PDF
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.

The Transcreener® cGAMP cGAS assay is available in a fluorescence polarization (FP) readout, which provide a safe HTS-compatible alternative to cumbersome radioassay methods and are more sensitive and less subject to interference than other detection methods. The readout uses a far-red tracer to minimize compound interference and lower false-positive rates.

The Transcreener® cGAMP cGAS FP Assay (Prod. No. BBL-3024) is a far-red, competitive fluorescence polarization (FP) assay. Because the antibody is highly selective for cGAMP, the assay can be used to measure activity of the cyclic GMP-AMP synthase (cGAS) enzyme which converts ATP and GTP, to cGAMP. cGAS is a recently discovered enzyme that acts as a foreign DNA sensor that induces an immune response via activation of the stimulator of interferon genes (STING) receptor. By directly measuring cGAMP with a highly selective antibody, it is possible to assay the activity of cGAS while screening large compound libraries for inhibitors. This assay can be used to i) measure enzymatic activity of cGAS, ii) screen compound libraries for cGAS inhibitors, iii) quantify inhibitor potency or iv) determine onhibitor-cGAS residence time.

The Transcreener® cGAMP cGAS FP Assay is a simple one step homogenous detection assay with excellent data quality (Z’ ≥ 0.7) and signal (≥85 mP polarization shift) at cGAMP ranges between 0.5 μM and 5 μM.

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