Transcreener® cGAMP cGAS FP Assay
|Application Set||Compound Screening|
1000 assays/384wells (BBL-3024-1K)
10000 assays/384wells (BBL-3024-10K)
Note: The exact number of assays depends on enzyme reaction conditions.
|Sensitivity||Excellent data quality (Z’ ≥ 0.7) and signal (≥85 mP polarization shift) at cGAMP ranges between 0.5 μM and 5 μM.|
|Detection Type||Fluorescent polarization|
cGAMP Antibody, cGAMP ATTO 633 Tracer, Stop & Detect Buffer B, 10X, ATP, GTP, cGAMP, Interferon Stimulatory DNA
Note: The plates are not included, please see manual for material not provided but necessary for assay.
|Other Product Data||
Click here for Complete Information from the Original Manufacturer
Easy-to-Use (mix-and-read method). Simple homogenous biochemical assay. Direct detection of unlabeled cGAMP. Safe, non-radioactive method. HTS Compatible with 96, 384, and 1536-well plates. Robust Assay Z’ > 0.7 under initial velocity conditions.
|Declaration||Manufactured by BellBrook Labs.|
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-20°C|
|Handling Advice||Avoid freeze/thaw cycles.|
|Product Specification Sheet|
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.
The Transcreener® cGAMP cGAS assay is available in a fluorescence polarization (FP) readout, which provide a safe HTS-compatible alternative to cumbersome radioassay methods and are more sensitive and less subject to interference than other detection methods. The readout uses a far-red tracer to minimize compound interference and lower false-positive rates.
The Transcreener® cGAMP cGAS FP Assay (Prod. No. BBL-3024) is a far-red, competitive fluorescence polarization (FP) assay. Because the antibody is highly selective for cGAMP, the assay can be used to measure activity of the cyclic GMP-AMP synthase (cGAS) enzyme which converts ATP and GTP, to cGAMP. cGAS is a recently discovered enzyme that acts as a foreign DNA sensor that induces an immune response via activation of the stimulator of interferon genes (STING) receptor. By directly measuring cGAMP with a highly selective antibody, it is possible to assay the activity of cGAS while screening large compound libraries for inhibitors. This assay can be used to i) measure enzymatic activity of cGAS, ii) screen compound libraries for cGAS inhibitors, iii) quantify inhibitor potency or iv) determine onhibitor-cGAS residence time.
The Transcreener® cGAMP cGAS FP Assay is a simple one step homogenous detection assay with excellent data quality (Z’ ≥ 0.7) and signal (≥85 mP polarization shift) at cGAMP ranges between 0.5 μM and 5 μM.