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BellBrook
AptaFluor SAH Methyltransferase TR-FRET Assay
CHF 0.00
In stock
BBL-3023-B100 assaysCHF 578.00
BBL-3023-1K1000 assaysCHF 1'649.00
BBL-3023-10K10000 assaysCHF 8'845.00
BBL-3023-100K10 x 10000 assaysINQ
Figure 1: Schematic overview of AptaFluor SAH Methyltransferase TR-FRET Assay.
| Product Details | |
|---|---|
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | All |
| Quantity |
100 assays/96wells (BBL-3023-B) 1000 assays/384wells (BBL-3023-1K) 10000 assays/384wells (BBL-3023-10K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Sensitivity | Excellent Z’ under a variety of assay conditions. |
| Range | Accommodates SAM concentrations from 100 nM to 5 μM. |
| Detection Type | TR-FRET |
| Kit Contains |
SAM, SAH, Enzyme Stop Reagent 10X, SAH Detection Buffer 10X, P1-Biotin, P2-Dylight 650, Tb-Streptavidin, Conjugation Buffer 1X Note: The plates are not included, please see manual for material not provided but necessary for assay. |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer
Easy-to-Use (mix-and-read method).Simple homogenous biochemical assay. Direct detection of UDP eliminates laborious coupling steps. HTS Compatible with 96, 384, and 1536-well plates. Outstanding deck and signal stability. Excellent sensitivity. Universal assay, usable with virtually any methyltransferase. Ultra-sensitive. Use with physiological SAM concentrations. Stable reagents. Robust assay! |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Epigenetic regulation has been shown to be a contributing factor in a variety of diseases. The discovery of methyltransferase inhibitors is currently an area of intense activity. Methyltransferase enzymes methylate a variety of substrates using S-adenosylmethionine (SAM) as the methyl donor leaving the common product S-adenosylhomocysteine (SAH).
The AptaFluor SAH Methyltransferase TR-FRET Assay (Prod. No. BBL-3023) is a universal assay for enzymes that convert S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH), with a far-red, time-resolved Försterresonance-energy-transfer (TR-FRET) readout. The assay can be used for all enzymes within the histone methyltransferase (HMT) and DNA methyltransferase (DNMT) families. The assay is designed specifically for high-throughput screening (HTS), with a simple mix-and-read format and 2 liquid addition steps. It is run in an endpoint mode: methyltransferase (MT) enzyme reactions are quenched with a universal stop reagent (provided) and then the detection reagents are added. The assay offers reagent stability and compatibility with commonly used multimode plate readers. The exquisite affinity and selectivity of the riboswitch combined with a positive TR-FRET signal enable screening and profiling of methyltransferases with unparalleled sensitivity. Far-red tracer further minimizes interference from fluorescent compounds and light scattering.
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