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BellBrook
Transcreener ADPR FP Assay
CHF 0.00
In stock
BBL-3030-1K1000 assaysCHF 1'125.00
BBL-3030-10K10000 assaysCHF 5'699.00
BBL-3030-100K10 x 10000 assaysINQ
Figure 1: Schematic overview of Transcreener ADPR FP Assay.
| Product Details | |
|---|---|
| Synonyms | ADP-ribose |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Specificity |
Outstanding selectivity of coupling enzyme for ADPR vs. NAD+, polyADPR and other nucleotides. |
| Crossreactivity | All |
| Quantity |
1000 assays/384wells (BBL-3030-1K) 10000 assays/384wells (BBL-3030-10K) 100000 assays/384wells (BBL-3030-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Range | Accommodates ADPR concentrations ranging from 10 nM up to 50 μM. |
| Detection Type | Fluorescent polarization |
| Kit Contains |
AMP2/GMP2 Antibody AMP2/GMP2 AlexaFluor 633 Tracer ADPR-AMP Coupling Enzyme Stop & Detect Buffer B, 10X ADPR Note: The plates are not included. An entirely black plate with a non-binding surface is required. We suggest Corning 384-well plates #4514; sold under #BBL-2229 or BBL-2230). |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.
The Transcreener ADPR Assay is a biochemical assay that measures the production of ADPR (also known as ADP-ribose) in enzymatic reactions. It is designed specifically for high throughput screening (HTS), with a single-addition, mix-and-read format. As examples, this assay can be used to detect the activity of CD38 or PARG, both of which may have implications for several therapeutic indications.
The ADPR assay is easy to integrate into automated HTS workflows, with outstanding reagent stability, sensitive detection of ADPR down to 10 nM and as high as 50 μM, and compatibility with commonly used multimode plate readers.
The Transcreener ADPR Assay measures enzymatic activity by using a coupling enzyme to convert ADPR into AMP. AMP is then detected using a proven Transcreener Assay format. By detecting ADPR in a homogenous format, the assay can be used with virtually any enzyme that produces ADPR. Transcreener ADPR technology uses an antibody selective to AMP and a far-red fluorescent tracer. As AMP is produced, it competes with the tracer changing the fluorescent properties and providing a fluorescent readout. The assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.
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