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BellBrook
MDA5 ATPase TR-FRET Assay System
CHF 0.00
In stock
BBL-3036-TR-1K1000 assaysCHF 1'010.00
BBL-3036-TR-10K10000 assaysCHF 3'418.00
BBL-3036-TR-100K10 x 10000 assaysINQ
NEW
Figure 1: Schematic Overview of the MDA5 ATPase Assay System with the Transcreener ADP2 Assays. For FP readout (A): ADP produced by the ATPase activity of MDA5 displaces an Alexa Fluor® 633 Tracer from the ADP2 antibody, resulting in decreased fluorescence polarization. For TRFRET readout (B): ADP produced by the ATPase activity of MDA5 displaces a HiLyte647 Tracer from the ADP2 Antibody conjugated to terbium (Tb), resulting in a decrease in TR-FRET. For FI readout (C): ADP produced by the ATPase activity of MDA5 displaces an Alexa Fluor® 594 Tracer from the ADP2 Antibody conjugated to an IRDye® QC-1 quencher, resulting in an increase in fluorescence intensity.
| Product Details | |
|---|---|
| Synonyms | Helicard; Melanoma Differentiation-associated Gene 5; IFIH1; Interferon Induced with Helicase C Domain 1 |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | Human |
| Quantity |
1000 assays/384wells (BBL-3036-TR-1K) 10000 assays/384wells (BBL-3036-TR-10K) 100000 assays/384wells (BBL-3036-TR-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Detection Type | TR-FRET |
| Kit Contains |
MDA5 Enzyme |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer |
| Declaration | Manufactured by BellBrook Labs. |
| Accession Number | Q9BYX4 |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The MDA5 Assay System is a tool that will simplify and advance exploration of novel innate immunity modulators. It is designed to screen and profile diverse compound libraries in a high-throughput format to identify small molecule inhibitors. It is intended for use with the trusted Transcreener ADP2 Assay (sold separately). The Transcreener ADP2 Assay is the only commercially available ADP assay that directly detects ADP production, i.e. without the use of coupling enzymes. The direct detection format minimizes compound interference, producing trustworthy results with Z' values greater than 0.7.
The assay is an extensively validated ADP detection method with use in over millions of wells by pharma.
When purchasing the MDA5 assay system, it is important to choose the assay system readout corresponding to the TR-FRET configuration of your Transcreener ADP2 Assay Kit!
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