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BellBrook
Transcreener pADPr PARP TR-FRET Assay
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BBL-3044-1K1000 assaysCHF 1’310.00
BBL-3044-10K10000 assaysCHF 6’269.00
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Figure 1: Schematic overview of Transcreener pADPr PARP TR-FRET Assay.
| Product Details | |
|---|---|
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | All |
| Quantity |
1'000 assays/384wells (BBL-3044-1K) 10'000 assays/384wells (BBL-3044-10K) 100'000 assays/384wells (BBL-3044-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Sensitivity | Excellent data quality (Z’ ≥ 0.7) |
| Range | Ranging from 10nM to 100nM. |
| Detection Type | TR-FRET |
| Kit Contains |
AMP2/GMP2 Antibody-Tb Note: The plates are not included, please see manual for material not provided but necessary for assay. |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer
Easy-to-Use (mix-and-read method). Detection of unlabeled pADPr produced by PARPs. Non-radioactive, safe assay method. HTS Compatible with 96, 384, and 1536-well plates. Robust Assay Z’ > 0.7 under initial velocity conditions. Far-red fluorescent readouts minimize compound interference. |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.
The Transcreener® pADPr PARP TR-FRET Assay is a biochemical HTS assay for measuring the production of poly(ADP-ribose) (pADPr) in poly(ADP-ribose) polymerase (PARP) reactions. The assay relies on pADPr Coupling Enzymes (CE) to convert pADPr into AMP, which is then detected using a far-red, timeresolved Förster-resonance-energy-transfer (TR-FRET) readout. As an example, the Transcreener pADPr PARP TR-FRET assay can be used to detect the activity of human PARP1, which uses NAD+ to form pADPr on itself, histones, and DNA repair proteins.
The Transcreener pADPr PARP TR-FRET assay is designed specifically for high throughput screening (HTS), with a single-addition, mix-and-read format. It is easy to integrate into automated HTS workflows, with outstanding reagent stability (deck stability > 16 hours, signal stability > 24 hours), robust detection of ADPR monomers released from pADPr over a range of down to 10 nM and as high as 100 μM, and compatibility with commonly used multimode plate readers. Data quality is excellent (Z’ ≥ 0.7), and the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.
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