BellBrook

PARP1 (human) (rec.) (active) (2µg)

As low as CHF 441.00

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BBL-2322-C0022 µgCHF 441.00

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Product Details
Synonyms	Poly(ADP-ribose) Polymerase 1; ADP-ribosyltransferase Diphtheria Toxin-like 1; NAD(+) ADP-ribosyltransferase 1; ARTD1; PARP-1
Product Type	Protein
Properties
Source/Host	Baculovirus system
Sequence	Active, full-length human PARP1 (aa 1-1014) protein with an N-terminal FLAG-tag.
Crossreactivity	Human
Biological Activity	The enzyme has been thoroughly validated with the Transcreener pADPr Assay Kit.
MW	115.1 kDa
Purity	≥80% (SDS-PAGE)
Concentration	0.1 mg/mL, 868 nM (the exact concentration may vary from batch to batch.)
Accession Number	P09874
Formulation	Liquid in 25 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Other Product Data	Uniprot P09874: PARP1 HUMAN
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-20°C
Long Term Storage	-80°C
Handling Advice	Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -80°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-γ to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

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