LEAP-2 ELISA Kit

LEAP-2, or Liver-Expressed Antimicrobial Peptide 2, plays a significant role in human health and disease. As an antimicrobial peptide, LEAP-2 is essential for the innate immune system, acting as a natural defense against various microbial threats, including bacteria, fungi and viruses. It also functions as an antagonist of ghrelin, a peptide hormone that stimulates appetite and regulates energy balance. In addition, LEAP-2 helps regulate inflammatory responses and maintain the balance of the intestinal microbiota, thus promoting overall gastrointestinal health. Understanding the function and regulation of LEAP-2 is crucial for developing new therapeutic strategies against infectious diseases, inflammatory conditions and gastrointestinal disorders. LEAP-2 could also serve as a biomarker for certain diseases, facilitating diagnosis and prognosis. Research on LEAP-2 is therefore essential to deepen our understanding of its role in health and disease, with important implications for clinical practice and public health interventions. The enzymatic immunoassay (ELISA) is based on the competition between unlabelled LEAP-2 and Biotin labelled LEAP-2 for limited specific rabbit anti-LEAP-antiserum sites. The complex rabbit antiserum LEAP-2 (free LEAP-2 or labelled) binds to the mouse monoclonal anti-rabbit antibody coated in the well. After the washing step, the complex binds to the mouse monoclonal anti-rabbit antibody and is revealed by Streptavidine_AChE (Tracer). Finally, Ellman's reagent (enzymatic substrate for AChE and chromogen) is added to the wells. The AChE tracer acts on the Ellman's Reagent to form a yellow compound that strongly absorbs at 414 nm. The intensity of the color, determined by spectrophotometry, is proportional to the amount of tracer bound to the well and is inversely proportional to the amount of free LEAP-2 present in the well during the immunological incubation.