Insulin (human) ELISA Kit

Insulin in a polypeptide composed of 51 amino acids divided into two chains (A and B) linked by disulfide bonds. Both chains are organized as alpha helix. Insulin is produced by the beta cells of the pancreatic islets in form of a precursor (prepro-insulin) and is modified to obtain the pro-insulin (peptide signal elimination) and finally insulin (peptide C elimination). The main function of the insulin is the regulation of the glucose concentration in the organism. The dysregulation of the production of insulin induced many disorders including diabetes. Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones�) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence). Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.