IFN-α 1 (pig) ELISA Kit

The IFN-α is one of the member of the type I interferons (IFN)s family and it is mainly involved in innate immunity. IFN-α 1 is secreted by immune (lymphocytes, NK cells, B-cells and T-cells, macrophages) and non-immune cells (fibroblasts, endothelial cells, osteoblasts and others) in answer to a viral infection. The main function of the IFN-α 1 is to alert the organism in case of viral infection by detection of abnormal double stranded DNA, but also to inhibit virus multiplication by action on the translation in infected cells. An abnormal production of IFN-α 1 induces immune dysfunction such as autoimmune diseases (systemic lupus erythematosus, rheumatoid arthritis...) or mediates tissue inflammation. Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones�) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence). Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.