IL-10 (pig) ELISA Kit

Also known as cytokine synthesis inhibitory factor (CSIF), IL-10 is a polypeptide of 157 amino acids glycosylated. The mature IL-10 is an homodimer linked by two intrachain disulfi de bridges (each sub-unit is 157 aa). IL-10 is produced by innate and non-innate cells, it is a cytokine of the innate and adaptive immunity. The mammalian cells known to secrete IL-10 are NK cells, cytotoxic T cells, memory Th1 and Th2 cells, macrophages monocytes, B cells, dendritic cells, hepatic stellates, keratinocytes, fetal erythroblasts. IL-10 is a cytokine with multiple pleiotropic effects in immunoregulation and inflammation. IL-10 is characterized like anti-infl ammatory cytokine: it represses the expression of inflammatory cytokines, such as Tumor Necrosis Factor-alpha (TNF-α), Interleukin-6 (IL-6) and Interleukin-1 (IL-1β), in macrophage. IL-10 is an immunoregulation cytokine and negative regulate Th1 responses by downmodulating antigen presenting capacity of APC. Due its central function, a dysregulation of the production of the lL-10 is implicated in asthma or autoimmune diseases. IL-10 is currently studied in immunooncology. Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones�) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence). Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.