IL-8 (pig) ELISA Kit

IL-8 is a polypeptide of 72-77 amino acids(1), known also as CXCL8, GCP-1 and NAP-1. Member of the CXC chemokine family, it can adopt a monomeric or homodimeric conformation containing a triple-stranded anti-parallel beta-sheet arranged in a Greek key and a long C-terminal helix. IL-8 could also form a heterodimer with PF4 activity. The receptors of IL-8 are CXCR1 and CXCR2. IL-8 is mainly produced by macrophages. It acts as an angiogenic factor in human microvascular endothelial cells. The main role of IL-8 in inflammation is the recruitment of neutrophils, although it is also responsible for the chemotactic migration and activation of monocytes, lymphocytes, basophils, and eosinophils at sites of inflammation. Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence). Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.