IL-13 (pig) ELISA Kit

Interleukin 13 (IL-13) is a nonglycosylated cytokine of 132 amino acids with a molecular weight of 12kDa and structured with four alpha-helices. IL-13 is secreted by T helper cell subsets, CD8+ T cells, mast cells, eosinophils and basophils. It has 25 % of sequence homology with IL-4, they share their actions on mononuclear phagocytic cells, endothelial cells, epithelial cells, and B cells. Both are key factors in the development of allergic hypersensitivity, and they also play a major role in exacerbating allergic and asthmatic symptoms. IL-13 is more importantly implicated as a central mediator of the physiologic changes induced by allergic inflammation in many tissues. IL-13 is the central mediator of allergic asthma, where it regulates eosinophilic inflammation, mucus secretion, and airway hyperresponsiveness. It could play a role in the resistance of some intracellular parasitis by down regulated of pro-inflammatory cytokines. Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones�) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence). Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.