Bertin Bioreagent

CGRP Affinity Sorbent

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BRT-A19482-11 eaCHF 268.00
 
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Product Details
Synonyms Calcitonin Gene-related Peptide Affinity Sorbent
Product Type Monoclonal Antibody
Properties
Application The following is the purification protocol to generate CGRP-free biological samples. i) Put 1 mL of sorbent in a tube. ii) Add up to 20 mL of plasma or biological sample. iii) Let it incubate overnight at +4°C with gentle agitation. iv) Pour the mixture in an empty column (around 10 x 1 cm) with a filter at the end. v) Remove the bottom cap and let the plasma or biological sample elute entirely (the gel should become dry but not for a long time). vi) The eluted plasma or biological sample is now free of CGRP and ready to be used to dilute standards and samples in the assay.
Other Product Data

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Declaration Manufactured by Bertin Bioreagent
Shipping and Handling
Shipping BLUE ICE
Long Term Storage +4°C
Use/Stability Stable for at least 1 year after receipt when stored at +4°C.
Documents
Product Specification Sheet
Datasheet Download PDF
Description
The CGRP Affinity Sorbent is an immobilized mouse monoclonal anti-CGRP antibody on a matrix resin. This product is designed for use in preparing CGRP-free plasma, which can be used as a sample matrix blank (SMB) for diluting standards and samples prior to analysis by EIA. The vial contains 1mL of sorbent which can treat up to 20mL sample volume. The use of sorbent will provide you with CGRP-free plasma/serum (pool 4 to 5 plasmas/serums together in order to decrease the variability between animals), and then reconstitute the CGRP standards and QC with free-CGRP serum.
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