Angiotensin II Inhibition Cocktail

The Angiotensin II (AII) Inhibition Cocktail has been developped in order to fix physiological concentration of Angiotensin II in biological samples namely blood. Indeed, it has been observed that sample preparation is highly recommanded when dosing Angiotensin II for preventing the formation or degration of Angiotensin II in vitro. Mix these solutions vial A and vial B to obtain the inhibitor cocktail ready to use. This solution has to be diluted 1:33 directly into the blood sample (i.e. for 1 mL of sample, add 30 μL of inhibition cocktail). The total volume is for approximately one sample of 10 mL of blood. After adding the inhibition cocktail, mix the tube and centrifuge at 3000g for 15 minutes at +4�C. Important notes: Plasma has to be prepared less than 30 minutes after collecting blood. Once mixed, the inhibition cocktail should be used immediately. Mix content of vial B into vial A (maximum volume of vial A is 10 mL) to obtain the ready-to-use 33x inhibition cocktail solution. Flocculation may appear.