10-Acetyl-3,7-dihydroxyphenoxazine

CHF 242.00
In stock
CDX-A0022-M02525 mgCHF 242.00
CDX-A0022-M200200 mgCHF 1'534.00
More Information
Product Details
Synonyms Amplex® Red (Registered trademark of LifeTechnologies)
Product Type Chemical
Properties
Formula

C14H11NO4

MW 257.24
CAS 119171-73-2
Source/Host Chemicals Synthetic.
Purity Chemicals ≥98% (HPLC)
Appearance Solid.
Solubility Soluble in DMSO or DMF.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key PKYCWFICOKSIHZ-UHFFFAOYSA-N
Smiles CC(=O)N1C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

10-acetyl-3,7-dihydroxyphenoxazin (Amplex Red) is a non-fluorescent, highly sensitive and stable probe for H2O2. Amplex Red is an applicable as a fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stechiometry with H2O2 to produce highly fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent allows researchers to detect the activity of many different enzymes. 10-acetyl-3,7-dihydroxyphenoxazin has absorption and fluorescence emission maxima of approximately 563 nm and 587 nm, respectively, and because its extinction coefficient is high (54,000 cm-1M-1), the assay can be performed either fluorometrically or spectrophotometrically.

Product References

(1) I. Snyrychova et al.; Phys. Plantarum, 135(1), 1-18 (2009) | (2) J.G. Montay et al.; J. Immunol. Meth. 202, 133 (1997) | (3) M. Zhou et al.; Anal. Biochem. 253, 162 (1997)

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