|Synonyms||4-Amino-5-methylamino- 2',7'-difluorofluorescein; Diaminofluorescein-FM|
|Purity Chemicals||≥98% (HPLC)|
|Solubility||Soluble in DMSO.|
|Identity||Determined by 1H-NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||Protect from light and moisture.|
|Use/Stability||Stable for at least 2 years after receipt when stored at -20°C.|
|Product Specification Sheet|
DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.
(1) W.S. Kim et al.; Anal. Chem. 78(6), 1859 (2006) | (2) M.M. Knight et al.; Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) | (3) H. Kojima et al.; Adv. Mat. 12(10), 763 (2002) | (4) H. Kojima et al.; Angew. Chem. 111( 21), 3419 (1999)