DAF-FM DA Solution
|Synonyms||4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate; Diaminofluorescein-FM diacetate|
|Purity Chemicals||≥98% (HPLC)|
|Appearance||Clear colourless liquid.|
|Solubility||Soluble in DMSO.|
|Concentration||5mM in DMSO (1mg in 0.40ml DMSO).|
|Identity||Determined by NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Keep cool and dry.
Protect from light and moisture.
|Use/Stability||Stable for at least 2 years after receipt when stored at -20°C.|
|Product Specification Sheet|
DAF-FM diacetate is a sensitive cell-permeable fluorescent probe important reagent for quantification of low concentrations of nitric oxide in solution. More photo-stable and less pH sensitive than DAF-2.This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM diacetate permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument suitable for detecting fluorescein.
(1) J. S. Carroll et al.; Anal. Chem. 79(14), 5133 (2007) | (2) Y. Itoh et al.; Anal. Biochem. 287(2), 203 (2000) | (3) H. Kojima et al.; Angew. Chem. 111(219), 3419 (1999)