DAF-FM DA Solution

CHF 574.00
In stock
CDX-A0524-M0011 mgCHF 574.00
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Product Details
Synonyms 4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate; Diaminofluorescein-FM diacetate
Product Type Chemical
Properties
Formula C25H18F2N2O7
MW 496.42
CAS 254109-22-3
Source/Host Chemicals Synthetic.
Purity Chemicals ≥98% (HPLC)
Appearance Clear colourless liquid.
Solubility Soluble in DMSO.
Concentration 5mM in DMSO (1mg in 0.40ml DMSO).
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key BEVHTVRRVVEMEF-UHFFFAOYSA-N
Smiles CNC1=C(N)C2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=C(F)C(OC(C)=O)=C3)C=C(OC(C)=O)C(F)=C2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

DAF-FM diacetate is a sensitive cell-permeable fluorescent probe important reagent for quantification of low concentrations of nitric oxide in solution. More photo-stable and less pH sensitive than DAF-2.This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM diacetate permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument suitable for detecting fluorescein.

Product References

(1) J. S. Carroll et al.; Anal. Chem. 79(14), 5133 (2007) | (2) Y. Itoh et al.; Anal. Biochem. 287(2), 203 (2000) | (3) H. Kojima et al.; Angew. Chem. 111(219), 3419 (1999)

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