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Chemodex
DAF-FM DA Solution
611
CHF
CHF 611.00
In stock
CDX-A0524-M0011 mgCHF 611.00
| Product Details | |
|---|---|
| Synonyms | 4-Amino-5-methylamino- 2',7'-difluorofluorescein diacetate; Diaminofluorescein-FM diacetate |
| Product Type | Chemical |
| Properties | |
| Formula | C25H18F2N2O7 |
| MW | 496.42 |
| CAS | 254109-22-3 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥98% (HPLC) |
| Appearance | Clear colourless liquid. |
| Solubility | Soluble in DMSO. |
| Concentration | 5mM in DMSO (1mg in 0.40ml DMSO). |
| Identity | Determined by NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | BEVHTVRRVVEMEF-UHFFFAOYSA-N |
| Smiles | CNC1=C(N)C2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=C(F)C(OC(C)=O)=C3)C=C(OC(C)=O)C(F)=C2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
DAF-FM diacetate is a sensitive cell-permeable fluorescent probe important reagent for quantification of low concentrations of nitric oxide in solution. More photo-stable and less pH sensitive than DAF-2.This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM diacetate permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument suitable for detecting fluorescein.
Product References
(1) J. S. Carroll et al.; Anal. Chem. 79(14), 5133 (2007) | (2) Y. Itoh et al.; Anal. Biochem. 287(2), 203 (2000) | (3) H. Kojima et al.; Angew. Chem. 111(219), 3419 (1999)
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