DAF-FM Solution

CHF 574.00
In stock
CDX-A0523-M0011 mgCHF 574.00
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Product Details
Synonyms Diaminofluorescein-FM; 4-Amino-5-methylamino-2',7'-difluorofluorescein
Product Type Chemical
Properties
Formula C21H14F2N2O5
MW 412.34
CAS 254109-20-1
Source/Host Chemicals Synthetic.
Purity Chemicals ≥98% (HPLC)
Appearance Clear colourless to yellow liquid.
Solubility Soluble in DMSO.
Concentration 5mM in DMSO (1mg in 0.49ml DMSO).
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key BJTLSPOVXMBXRZ-UHFFFAOYSA-N
Smiles CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm and a dectection limit of 3 nM, DAF-FM can be detected using any instrument that can be used to detect fluorescein. It is more photo-stable and less pH sensitive than DAF-2.

Product References

(1) W.S. Kim et al.; Anal. Chem. 78(6), 1859 (2006) | (2) M.M. Knight et al.; Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) | (3) H. Kojima et al.; Adv. Mat. 12(10), 763 (2002) | (4) H. Kojima et al.; Angew. Chem. 111( 21), 3419 (1999)

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