Chemodex

DAF-FM Solution

CHF 611.00

In stock

CDX-A0523-M0011 mgCHF 611.00

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Product Details
Synonyms	Diaminofluorescein-FM; 4-Amino-5-methylamino-2',7'-difluorofluorescein
Product Type	Chemical
Properties
Formula	C₂₁H₁₄F₂N₂O₅
MW	412.34
CAS	254109-20-1
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥98% (HPLC)
Appearance	Clear colourless to yellow liquid.
Solubility	Soluble in DMSO.
Concentration	5mM in DMSO (1mg in 0.49ml DMSO).
Identity	Determined by NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	BJTLSPOVXMBXRZ-UHFFFAOYSA-N
Smiles	CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Keep cool and dry. Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm and a dectection limit of 3 nM, DAF-FM can be detected using any instrument that can be used to detect fluorescein. It is more photo-stable and less pH sensitive than DAF-2.

Product References

(1) W.S. Kim et al.; Anal. Chem. 78(6), 1859 (2006) | (2) M.M. Knight et al.; Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) | (3) H. Kojima et al.; Adv. Mat. 12(10), 763 (2002) | (4) H. Kojima et al.; Angew. Chem. 111( 21), 3419 (1999)

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