|Purity Chemicals||≥98% (HPLC)|
|Solubility||Soluble in DMSO.|
|Identity||Determined by NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Keep cool and dry.
Protect from light and moisture.
|Use/Stability||Stable for at least 2 years after receipt when stored at -20°C.|
|Product Specification Sheet|
7-Azido-4-methylcoumarin is a highly sensitive and selective fluorogenic H2S probe. The aromatic azide moiety of AzMC is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with λex = 365 nm and λem = 450 nm. It is a photoaffinity labeling probe for the substrate binding site of human sulfotransferase 1A1 (SULT1A1). 7-Azido-4-methylcoumarin is a tool for monitoring the activity of pyridoxal-5'-phosphate (PLP)-dependent enzymes (e.g. cystathionine β-synthase (CBS), cystathionine γ-lyase (CGL) and tryptophan synthase (TS)) and to identify novel cystathionine β-synthase (CBS) inhibitors and activators.
(1) G. Chen, et al.; Protein Sci. 8, 2151 (1999) | (2) M.K. Thorson, et al.; Angew. Chem. Int. Ed. Engl. 52, 4641 (2013) | (3) B. Chen, et al.; Analyst. 138, 946 (2013)