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Chemodex
4'-(Aminomethyl)fluorescein hydrochloride
CHF 0.00
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| Product Details | |
|---|---|
| Synonyms | 4-AMF; 4'-((Aminoacetamido)methyl)fluorescein |
| Product Type | Chemical |
| Properties | |
| Formula | C21H16ClNO5 |
| MW | 397.81 |
| CAS | 91539-64-9 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥97% (HPLC) |
| Appearance | Orange solid. |
| Solubility | Soluble in DMSO. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | JJYRABHKUBHHSU-UHFFFAOYSA-N |
| Smiles | OC1=CC=C(C(C2=CC=CC=C2C(O)=O)=C(C=C3)C(O4)=C(CN)C3=O)C4=C1.Cl |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
4'-(Aminomethyl)fluorescein is an excellent building block for developing fluoresceinated bioconjugates. Its amino group readily reacts with activated carboxy esters (such as NHS ester). The primary aliphatic amine of 4'-(aminomethyl)fluorescein can be reversibly coupled to aldehydes and ketones to form a Schiff base, which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNBH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC. Spectral Data: λex 494 nm; λem 520 nm.
Product References
(1) M.T. Shipchandler, et al.; Anal. Biochem. 162, 89 (1987) | (2) R. Dhar Dubey, et al.; Nanotheranostics 1, 59 (2017)