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Chemodex
Bromphenol blue
As low as
110
CHF
CHF 110.00
In stock
Only %1 left
CDX-B0026-G02525 gCHF 110.00
NEW
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Bromophenol blue; 3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein; NSC 7818; BPB 670; Albutest |
| Product Type | Chemical |
| Properties | |
| Formula | C19H10Br4O5S |
| MW | 669.96 |
| CAS | 115-39-9 |
| RTECS | SJ7453000 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥95% (Dye content) |
| Appearance | Faint beige to orange to red powder. |
| Solubility | Soluble in DMF (30mg/ml), DMSO (30mg/ml) or ethanol (10mg/ml). Slightly soluble in aqueous buffer (1mg/ml). |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | UDSAIICHUKSCKT-UHFFFAOYSA-N |
| Smiles | O=S1(=O)OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C=4C=CC=CC41 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +20°C |
| Long Term Storage | +20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at RT. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Scientific Background Information
Product Description
Bromophenol blue is a widely used pH indicator dye and tracking dye for biochemical and analytical applications. It exhibits a distinct color change over its transition range and is commonly employed in acid-base titrations, with a transition range of pH 3 (yellow) to 4.6 (purple), electrophoresis sample loading buffers, and laboratory staining protocols. Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier. Spectral Data: λmax=610 nm. Bromophenol blue is an essential reagent for research laboratories, offering reliable performance in molecular biology, biochemistry, and analytical chemistry.
Product-specific References
(1) P. Harris & D.J. Mazia; Biophys. Biochem. Cytol. 5, 343 (1959) | (2) D.W. Menzies; Stain Technol. 36, 285 (1961) | (3) G.D. Burford & B.T. Pickering; Biochem. J. 128, 941 (1972) | (4) R. Flores; Anal. Biochem. 88, 605 (1978) | (5) S. Dhananasekaran, et al.; J. Adv. Res. 7, 113 (2016) | (6) A.Z. Dangui, et al.; Spectrochim. Acta A Mol. Biomol. Spectrosc. 203, 333 (2018) | (7) C. Burel, et al.; Lett. Appl. Microbiol. 72, 358 (2021)