Biotin-(5-fluorescein)-conjugate

CHF 77.00
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CDX-B0078-M0011 mgCHF 77.00
CDX-B0078-M0055 mgCHF 306.00
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Product Details
Synonyms Biotin-4-Fluorescein; B4F; N-(Biotinylamidoethyl)-fluorescein-5-carboxamide; N-(Fluorescein-5-carbamidoethyl)biotinamide
Product Type Chemical
Properties
Formula C33H32N4O8S
MW 644.70
CAS 957494-27-8
Source/Host Chemicals Synthetic.
Purity Chemicals ≥90% (HPLC)
Appearance Yellow solid.
Solubility Soluble in DMSO.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key DFUFXKZUEOKPSD-LFERIPGTSA-N
Smiles O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: λex 490nm; λem 524nm in 0.1M Tris pH8.0.

Product References

(1) G. Kada et al. Biochim. Biophys. Acta 1427, 33 (1999) | (2) G. Kada et al. Biochim. Biophys. Acta 1427, 44 (1999) | (3) N. Humbert, et al.; Electrophoresis 26, 47 (2005) | (4) F.M. Aslan, et al.; PNAS 102, 8507 (2005) | (5) M.J. Waner & D.P. Mascotti; J. Biochem. Biophys. Methods 70, 873 (2008) | (6) A. Ebner, et al.; Methods Mol. Biol. 418, 73 (2008) | (7) R. Mittal & M.P. Bruchez; Bioconjug. Chem. 22, 362 (2011)

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