Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
Chemodex
Biotin-(5-fluorescein)-conjugate
81
CHF
CHF 81.00
In stock
CDX-B0078-M0011 mgCHF 81.00
CDX-B0078-M0055 mgCHF 326.00
| Product Details | |
|---|---|
| Synonyms | Biotin-4-Fluorescein; B4F; N-(Biotinylamidoethyl)-fluorescein-5-carboxamide; N-(Fluorescein-5-carbamidoethyl)biotinamide |
| Product Type | Chemical |
| Properties | |
| Formula | C33H32N4O8S |
| MW | 644.70 |
| CAS | 957494-27-8 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥90% (HPLC) |
| Appearance | Yellow solid. |
| Solubility | Soluble in DMSO. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | DFUFXKZUEOKPSD-LFERIPGTSA-N |
| Smiles | O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: λex 490nm; λem 524nm in 0.1M Tris pH8.0.
Product References
(1) G. Kada et al. Biochim. Biophys. Acta 1427, 33 (1999) | (2) G. Kada et al. Biochim. Biophys. Acta 1427, 44 (1999) | (3) N. Humbert, et al.; Electrophoresis 26, 47 (2005) | (4) F.M. Aslan, et al.; PNAS 102, 8507 (2005) | (5) M.J. Waner & D.P. Mascotti; J. Biochem. Biophys. Methods 70, 873 (2008) | (6) A. Ebner, et al.; Methods Mol. Biol. 418, 73 (2008) | (7) R. Mittal & M.P. Bruchez; Bioconjug. Chem. 22, 362 (2011)
Related Products
