6-Carboxy-4',5'-dichloro-2', 7'-dimethoxyfluorescein succinimidyl ester

Specifications / Handling

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Product Details
Synonyms	6-JOE, SE; 2,5-Dioxo-1-pyrrolidinyl 4’,5’-Dichloro-3’,6’-dihydroxy-2’,7’-dimethoxy-3-oxospiro[isobenzofuran-1(3H),9’-[9H]xanthene]-6-carboxylate
Product Type	Chemical
Properties
Formula	C₂₇H₁₇Cl₂NO₁₁
MW	602.34
CAS	113394-23-3
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥80% (TLC)
Appearance	Orange solid.
Solubility	Soluble in DMSO, DMF or methanol.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	UUVBVONAGUSCCH-UHFFFAOYSA-N
Smiles	O=C(C1=C2C=C(C(ON3C(CCC3=O)=O)=O)C=C1)OC42C5=C(C(Cl)=C(O)C(OC)=C5)OC6=C(Cl)C(O)=C(OC)C=C64
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Keep cool and dry. Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Amine-reactive fluorescent probe. A conventional fluorophore predominantly used in automated DNA sequencing. It is also used to label nucleotides. Characterized by an intermediate-wavelength spectra, high quantum yield, and low pH sensitivity in the physiological range. Has a similar absorption and emission spectra to those of rhodamine 6G. Spectral Data: λex 520nm, λem550 nm in Buffer pH 12; λex 520nm, λem 548nm in DMSO.

Product References

(1) F.F. Chehab & Y.W. Kann; PNAS 86, 9178 (1989) | (2) S. Carson, et al.; Anal. Chem. 65, 3219 (1993) | (3) S.C. Hung, et al.; Anal. Biochem. 238, 165 (1996) | (4) A.N. Glazer & R.A. Mathies; Curr. Opin. Biotechnol. 8, 94 (1997) | (5) M. Hahn, et al.; Electrophoresis 22, 2691 (2001) | (6) K. Petersen, et al.; Mol. Cell Probes 18, 117 (2004) | (7) I.M. Mackay; Clin. Microbiol. Infect. 3, 190 (2004) | (8) L.J. Kricka; Ann. Clin. Biochem. 39, 114 (2002)

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