Biotin-X-NHS

Specifications / Handling

More Information
Product Details
Synonyms	NHS-LC-Biotin; Biotinamidohexanoic acid N-hydroxysuccinimide ester; (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester; N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester; N-(+)-Biotinyl-6-aminohexanoic acid NHS ester; Enzotin
Product Type	Chemical
Properties
Formula	C₂₀H₃₀N₄O₆S
MW	454.54
CAS	72040-63-2
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥97% (NMR)
Appearance	White to off-white powder.
Solubility	Soluble in DMSO (20mg/ml), DMF(20mg/ml) or methanol.
Identity	Determined by ¹H-NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	UVGHPGOONBRLCX-NJSLBKSFSA-N
Smiles	O=C(NCCCCCC(ON1C(CCC1=O)=O)=O)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Biotin-X-NHS is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance. Biotin-X-NHS is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. The Biotin-X-NHS molecule is cell membrane permeable and can be used to label intracellular proteins. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.

Product References

(1) G.T. Hermanson; Bioconjugate Techn. 2nd p506-545 (2008)

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