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Chemodex
Hoechst 34580 Solution
258
CHF
CHF 258.00
In stock
CDX-B0448-L0055 mlCHF 258.00
| Product Details | |
|---|---|
| Synonyms | N,N-Dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]benzenamine trihydrochloride; HOE 34580; BisBenzimide H 34580 |
| Product Type | Chemical |
| Properties | |
| Formula |
C27H29N7 . 3HCl |
| MW | 560.95 |
| CAS | 911004-45-0 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥98% (HPLC) |
| Appearance | Liquid. |
| Solubility | Soluble in water. |
| Concentration | 1mg/ml in water |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | IPMZLKUUQSFUTA-UHFFFAOYSA-N |
| Smiles | C[NH+](C)C(C=C1)=CC=C1C(N2)=NC3=C2C=C(C4=NC5=C(C=C([NH+]6CC[NH+](C)CC6)C=C5)N4)C=C3.[Cl-].[Cl-].[Cl-] |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. The blue fluorescent Hoechst dye is a cell permeable nucleic acid stain that has multiple applications, including sensitive detection of DNA in the presence of RNA in agarose gels, automated DNA determination, sensitive determination of cell number and chromosome sorting. Useful vital stain for the flow cytometric recognition of DNA damage and other viability measurements by monitoring the emission spectral shifts of the dyes. Because this fluorescent stain labels DNA, it can also be used to visualize nuclei and mitochondria. Hoechst 34580 can be excited by violet laser at 405 nm, and emit blue/cyan fluorescence light around an emission maximum at ~440 nm. It is cell-permeable and an excellent tool for flow cytometry studies.
Product References
(1) S.A. Latt & G. Stetten: J. Histochem. Cytochem. 24, 24 (1976) | (2) D.P. Kelly, et al.: Aus. J. Chem. 47, 1751 (1994) | (3) W.G. Telford, et al.: Cytometry A. 54, 48 (2003) | (4) D.A. Evans & S. Neidle: J. Med. Chem. 49, 4232 (2006) | (5) W. Lei, et al.: ChemPhysChem 12, 2933 (2011)