Chemodex

5(6)-Carboxy-2',7'-difluorofluorescein

Name: 5(6)-Carboxy-2',7'-difluorofluorescein
Brand: Chemodex
SKU: CDX-C0262
Price: 103.00 CHF
Availability: InStock

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CDX-C0262-M05050 mgCHF 103.00

Specifications / Handling

More Information
Product Details
Product Type	Chemical
Properties
Formula	C₂₁H₁₂F₂O₇
MW	414.32
CAS	251450-64-3
Source/Host Chemicals	Synthetic
Purity Chemicals	≥95% (NMR)
Appearance	Red powder.
Solubility	Soluble in DMSO.
Identity	Determined by ¹H-NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	DDDAACFHCLQORF-UHFFFAOYSA-N
Smiles	OC(C(F)=C1)=CC2=C1C3(C(C=CC=C4)=C4C(O3)=O)C5=CC(F)=C(O)C=C5O2.CC(O)=O
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

5(6)-Carboxy-2',7'-difluorofluorescein is a fluorinated fluorescein used as a fluorescent dye used as a building block for synthesis of fluorescent probes. It is a replacement for the widely used carboxyfluorescein and fluorescein isothiocyanate since the absorption and emission are virtually unchanged. It can be excited with the 488nm spectral line of the argon-ion laser used in flow cytometers and confocal laser scanning microscopes. The lower pKa, increased resistance to photobleaching, high quantum yield, and diminished quenching on protein conjugation make the dyes superior to other fluoresceins for bioconjugation. Spectral data: Absoprtion 492nm, Emmision 516nm

Product-specific References

(1) W.-C. Sun, et al.; J. Org. Chem. 62, 6469 (1997) | (2) N.G. Norager, et al.; ACS Chem. Biol. 8, 2033 (2013)

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