BCECF-AM Solution

CHF 230.00
In stock
CDX-C0603-M0011 mgCHF 230.00
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Product Details
Synonyms 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid
Product Type Chemical
Properties
Formula C42H40O21
MW 808.7
CAS 117464-70-7
Source/Host Chemicals Synthetic.
Purity Chemicals ≥95% (HPCE)
Appearance Liquid.
Solubility Soluble in DMSO.
Concentration 5mM in DMSO (1mg in 0.25ml DMSO)
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key NTECHUXHORNEGZ-UHFFFAOYSA-N
Smiles O=C(C)OCOC1=C(CCC(OCOC(C)=O)=O)C=C(C2(C(C=CC(C(OCOC(C)=O)=O)=C3)=C3C(O2)=O)C(C(O4)=C5)=CC(CCC(OCOC(C)=O)=O)=C5OCOC(C)=O)C4=C1
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium. Spectral properties: λex 490nm; λem 535nm; λex=505nm; λem=520nm in DMSO.

Product References

(1) T.J. Rink, et al.; J. Cell Biol. 95, 189 (1982) | (2) C. Dive, et al.; Mol. Cell Probe 2, 131 (1988) | (3) A.K. Ho, et al.; Am. J. Physiol. 261, C642 (1991) | (4) W. Obexer, et al; Tropical Med. Parasit. 46(1), 45 (1995) | (5) S. Chow, et al.; Cytometry 24, 360 (1996) | (6) H. Urano, et al.; Chem. Lett. 12, 1217 (1997) | (7) L. Balkay, et al.; Cytometry 28, 42 (1997) | (8) P. Ozkan & R. Mutharasan; Biochim. Biophys Acta 1572, 143 (2002) | (9) N. O'Connor & R.B. Silver; Meth. Cell Biol. 81, 415 (2007)

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