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Chemodex
DAF-4 DA solution
530
CHF
CHF 530.00
In stock
CDX-D0516-M0011 mgCHF 530.00
| Product Details | |
|---|---|
| Synonyms | DAF-4 diacetate solution; 3,4-Diamino-2',7'-dichlorofluorescein diacetate solution |
| Product Type | Chemical |
| Properties | |
| Formula |
C24H16Cl2N2O7 |
| MW | 515.3 |
| CAS | 208850-34-4 (parent compound DAF-4) |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥90% (HPLC) |
| Appearance | Liquid. |
| Solubility | Soluble in chloroform or DMSO. |
| Concentration | 5mM in DMSO (1mg in 0.39ml DMSO). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | OOSGYDWTQGDKAY-UHFFFAOYSA-N |
| Smiles | ClC1=C(OC(C)=O)C=C(OC(C=C(OC(C)=O)C(Cl)=C2)=C2C34OC(C5=C4C=C(N)C(N)=C5)=O)C3=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
DAF-4 is a indicator for nitric oxide. As with other fluorescein diacetates (DAFs), the non-fluorescent DAF-4 diacetate (DAF-4 DA) is membrane permeant and is deacetylated by intracellular esterases to form DAF-4, which than can ract with NO to form the fluorescent DAF-4T. Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex=505nm; λem=530nm.
Product References
(1) H. Kojima, et al.; Anal. Chem. 70, 2446 (1998) | (2) N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998) | (3) T. Nagano; Luminescence 14, 283 (1999) (Review) | (4) T. Nagano; Proc. Jpn. Acad. Ser. B 86, 837 (2010) (Review)