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Chemodex
DAF-2 DA Solution
Product Details | |
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Synonyms | 4,5-Diaminofluorescein diacetate; 5,6-Diaminofluorescein diacetate |
Product Type | Chemical |
Properties | |
Formula | C24H18N2O7 |
MW | 446.41 |
CAS | 205391-02-2 |
Source/Host Chemicals | Synthetic. |
Purity Chemicals | ≥95% (HPLC) |
Appearance | Liquid. |
Solubility | Soluble in DMSO. |
Concentration | 5mM in DMSO (1mg in 0.45ml DMSO) |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | PTSUYDXEEKDBQU-UHFFFAOYSA-N |
Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=C(N)C(N)=C3)C1=CC=C(OC(C)=O)C=C1O2 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice |
Keep cool and dry. Protect from light. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet | Download PDF |
DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo. Spectral Data: λex 491nm; λem 513nm.
(1) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003) | (2) N. Nagata et al.; J. Biochem. 125(4), 658 (1999) | (3) H. Kojima et al.; Anal. Chem. 70(13), 2446 (1998) | (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998) | (5) H. Kojima et al.; Neuroreport 9, 3345 (1998)