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Chemodex
FURA 2-AM Solution (1mM in DMSO)
CHF 0.00
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| Product Details | |
|---|---|
| Synonyms | FURA-2 acetoxymethyl ester; FURA-2 pentakis(acetoxymethyl) ester |
| Product Type | Chemical |
| Properties | |
| Formula | C44H47N3O24 |
| MW | 1001.87 |
| CAS | 108964-32-5 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | Liquid. |
| Solubility | Soluble in DMSO. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | VPSRLGDRGCKUTK-UHFFFAOYSA-N |
| Smiles | O=C(OCOC(C)=O)C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C3 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep under inert gas. Protect from light and oxygen. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
The fluorescent probe FURA 2/AM is a membrane-permeable derivative of the calcium chelator FURA 2. Inside cells, FURA 2/AM is rapidly hydrolyzed by cytoplasmic esterases to FURA 2, which remains within the cell. Non-invasive, intracellular calcium indicator that is UV light-excitable. Fluorescent Ca2+ indicator, selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA 2/AM is used to determine calcium concentrations in many cellular systems, including: cardiac myocytes, vascular smooth muscle cells and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (λex = 362nm, λem = 512nm) differ from those during high calcium conditions (λex = 335nm, λem = 505nm). FURA 2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Spectral Data: λex 355nm; λem 495nm in 0.1 M Tris pH 8.0.
Product References
(1) G. Grynkiewicz, et al.; J. Biol. Chem. 260, 3440 (1985) | (2) I. Maruyama, et al.; FEBS Lett. 220, 89 (1987) | (3) M.E. Janconi, et al.; J. Cell Biol. 110, 1555 (1990) | (4) Y. Schwartz, et al.; Endocrinology 131, 772 (1992) | (5) G.H. Rao, et al.; Biochem. Med. Metab. Biol. 47, 226 (1992) | (6) M.J.Davis, et al.; Am. J. Physiol. 263, H1292 (1992) | (7) M.J. Davis, et al.; Am. J. Physiol. 263, H1292 (1992) | (8) M.L. Field, et al.; Cell Calcium. 16, 87 (1994) | (9) R.W. Sabnis; Handbook of biological dyes and stains (2010)