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Chemodex
IPB
Product Details | |
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Synonyms | 4-[3-(4-Iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate sodium salt |
Product Type | Chemical |
Properties | |
Formula | C19H10IN6NaO10S2 |
MW | 696.34 |
CAS | 161617-45-4 |
Source/Host Chemicals | Synthetic. |
Purity Chemicals | ≥90% (HPLC) |
Appearance | Pale yellow powder. |
Solubility | Soluble in chloroform. |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | LKVNWZJLLRMMAS-UHFFFAOYSA-M |
Smiles | [Na+].[O-][N+](=O)C1=CC=C(N2N=C(N=[N+]2C2=CC=C(I)C=C2)C2=CC=C(C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(=C1)[N+]([O-])=O |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet | Download PDF |
4-[3-(4-iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate sodium salt (IPB) have found application in clinical assays and cell proliferation assays that are based on a spectrophotometric measurement of dehydrogenase activities of cells. Disulfonated tetrazolium salt IPB, that produces a highly water-soluble formazan dye that proved to be extremely useful as a cell-viability indicator. IPB is reduced by reduced nicotinamide adenine dinucleotide (NADH) to formazan in the presence of a redox mediator such as 1-methoxy PMS, which provides the basis to specrophotometric assays of NADH.
(1) T. Mosmann; Immun. Meth. 65, 55 (1983) | (2) M.M. Nachlas et al.; Anal. Biochem. 1, 317 (1960)