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Chemodex
IPTG
50
CHF
CHF 50.00
In stock
CDX-I0030-G0011 gCHF 50.00
CDX-I0030-G0055 gCHF 204.00
CDX-I0030-G01010 gCHF 326.00
| Product Details | |
|---|---|
| Synonyms | Isopropyl β-D-1-thiogalactopyranoside; Isopropyl β-D-thiogalactoside |
| Product Type | Chemical |
| Properties | |
| Formula |
C9H18O5S |
| MW | 238.3 |
| CAS | 367-93-1 |
| Purity Chemicals | ≥99% (HPLC) |
| Appearance | White to off-white powder. |
| Solubility | Soluble in water (50 mg/ml), DMSO (20mg/ml) or ethanol (20mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
| Smiles | CC(S[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)C |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +20°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
IPTG is a molecular biology reagent. It is a β-Galactosidase activity inducer in several bacteria, by binding and inhibiting the lac repressor and it is used in gene cloning together with X-Gal, in a technique called blue/white screening. It is used in the concentration range of 100µM - 1.5mM to induce protein expression. IPTG distinguishes between recombinant and non-recombinant plasmids carrying the β-galactosidase gene.
Product References
(1) J.F. Rodriguez & G.L. Smith; Nucleic Acids Res. 18, 5347 (1990) | (2) J. Sambrook & D. Russell; Cold Spring Harbor Laboratory Press (2001) | (3) P. Howhan & S. Pornbanlualap; Science Asia 29, 347 (2003)