Luminol

Very efficient and commonly used chemiluminiscent compound in many applications. Cyclic diacyl hydrazide that exhibits chemiluminescence upon oxidation. Oxidizing agents capable of initiating this particular reaction are nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants. As an example, HRP combined with hydrogen peroxide as an activator, causes luminescent peroxidation of luminol. This reaction can be enhanced by certain phenol derivatives, such as p-substituted phenols. Luminol can also be oxidized and chemiluminesce, by compounds containing iron, copper, gold, and cyanide. Luminol is a reagent for the generation of chemiluminescence in biological assays including nitric oxide and myeloperoxidase assays or in fornensic blood tests. Spectral data: λex=355nm, λem=411nm.