D,L-Luciferin 6'-methyl ether

D,L-Luciferin 6′-methyl ether is an analog of Luciferin in which the 6′-hydroxyl group is methylated. This structural modification significantly reduces productive oxidation by firefly luciferase while retaining enzyme binding affinity. As a result, the compound functions as a non-productive substrate analog that can competitively interfere with D-luciferin-dependent bioluminescence. D,L-Luciferin 6′-methyl ether is used in mechanistic enzymology and chemical biology to investigate luciferase substrate recognition, active-site interactions, and structure-activity relationships. It is also valuable for the evaluation of engineered or mutant luciferases with altered substrate specificity. Spectral Data: Excitation max: ~ 326nm, Emission max: ~430nm (accord. Lit).