2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid sodium salt hydrate

Fluorogenic substrate used to measure sensitively enzymatic neuraminidase activity in fluorometric assays in mammalian and viral sources. Neuraminidase is catalyzed by the hydrolysation to form N-acetylneuraminic acid and the blue fluorescent soluble compound 4-Methylumbelliferone, which can be detected fluorometrically. Spectral data: λex 315 nm| λem 374 nm (pH.7.0) or λex 365 nm| λem 445 nm (after cleavage by neuraminidase). Used for fluorescent staining of sialidases in PAGE.