Chemodex

4-Methylumbelliferyl-β-D-galactopyranoside

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Product Details
Synonyms MUG; MUGA; MUGal; 4-Methylumbelliferyl-β-D-galactoside
Product Type Chemical
Properties
Formula C16H18O8
MW 338.31
CAS 6160-78-7
Source/Host Chemicals Synthetic
Purity Chemicals ≥98% (NMR)
Appearance White to off-white powder.
Solubility Soluble in DMSO.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

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Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key YUDPTGPSBJVHCN-DZQJYWQESA-N
Smiles O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@H](O)[C@@H]1O
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
Product Specification Sheet
Datasheet Download PDF
Description
4-Methylumbelliferyl-β-D-galactopyranoside (MUG) is a non-fluorescent but sensitive fluorogenic substrate for β-galactosidase (β-gal) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting galactosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is used as a substrate in β-galactosidase activity assay. Hydrolysis of MUG releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).
Product References
(1) A.J. Furth & D. Robinson; Biochem. J. 97, 59 (1965) | (2) G. Kominami, et al.; Clin. Chim. Acta 103, 381 (1980) | (3) H. Labrousse, et al.; J. Immunol. Methods 48, 133 (1982) | (4) H. Ohmori, et al.; J. Immunol. Methods. 147, 119 (1992) | (5) J.D. Berg, et al.; Appl. Environ. Microbiol. 54, 2118 (1998) | (6) F. Vidal-Aroca, et al.; Biotechn. 40, 433 (2006)
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