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Chemodex
4-Methylumbelliferyl-β-D-galactopyranoside
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CHF
CHF 110.00
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Product Details | |
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Synonyms | MUG; MUGA; MUGal; 4-Methylumbelliferyl-β-D-galactoside |
Product Type | Chemical |
Properties | |
Formula | C16H18O8 |
MW | 338.31 |
CAS | 6160-78-7 |
Source/Host Chemicals | Synthetic |
Purity Chemicals | ≥98% (NMR) |
Appearance | White to off-white powder. |
Solubility | Soluble in DMSO. |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | YUDPTGPSBJVHCN-DZQJYWQESA-N |
Smiles | O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](CO)[C@H](O)[C@@H]1O |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
Product Specification Sheet | |
Datasheet |
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Description
4-Methylumbelliferyl-β-D-galactopyranoside (MUG) is a non-fluorescent but sensitive fluorogenic substrate for β-galactosidase (β-gal) yielding a blue fluorescent solution. It is a fluorogenic MU substrate for detecting galactosidase in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It is used as a substrate in β-galactosidase activity assay. Hydrolysis of MUG releases the fluorescent product 4-MU. Spectral Data. λEx/λEm= 360/450 nm (cleaved product, pH-dependent).
Product References
(1) A.J. Furth & D. Robinson; Biochem. J. 97, 59 (1965) | (2) G. Kominami, et al.; Clin. Chim. Acta 103, 381 (1980) | (3) H. Labrousse, et al.; J. Immunol. Methods 48, 133 (1982) | (4) H. Ohmori, et al.; J. Immunol. Methods. 147, 119 (1992) | (5) J.D. Berg, et al.; Appl. Environ. Microbiol. 54, 2118 (1998) | (6) F. Vidal-Aroca, et al.; Biotechn. 40, 433 (2006)