Nitrotetrazolium blue chloride

CHF 61.00
In stock
CDX-N0009-M100100 mgCHF 61.00
CDX-N0009-G0011 gCHF 326.00
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Product Details
Synonyms NBT; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; Nitro BT
Product Type Chemical
Formula C40H30Cl2N10O6
MW 817.2
CAS 298-83-9
RTECS XF8045000
Source/Host Chemicals Synthetic.
Purity Chemicals ≥98% (HPLC)
Appearance Yellow powder.
Solubility Soluble in water.
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Smiles [Cl-].[Cl-].COC1=CC(=CC=C1[N+]1=NC(=NN1C1=CC=C(C=C1)[N+]([O-])=O)C1=CC=CC=C1)C1=CC=C(C(OC)=C1)[N+]1=NC(=NN1C1=CC=C(C=C1)[N+]([O-])=O)C1=CC=CC=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase). Well-known scavenger of superoxide anions. Dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP). This substrate system produces an insoluble NBT diformazan end product that is blue in color and can be observed visually. When used with BCIP, it is suitable for detection of alkaline phosphatase in western blots, for immunohistological staining procedures and for colorimetric indication of bacterial infection in blood samples. Used as a redox indicator for enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase (G6PDH), phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels and pentose shunt dehydrogenses. The NBT/BCIP reaction is also used for colorimetric/spectrophotometric activity assays of oxidoreductases. One application is in activity stains in gel electrophoresis, such as with the mitochondrial electron transport chain complexes.

Product References

(1) D.G. Nathan, et al.; J. Clin. Invest. 48, 1895 (1969) | (2) J. McGadey; Histochem. Histochem. Histochim. 23, 180 (1970) | (3) R. Freeman & B. King; J. Clin. Pathol. 25, 912 (1972) | (4) D.A. Knecht & R.L. Dimond; Anal. Biochem. 136, 180 (1984) | (5) Y. Nisimoto, et al.; J. Biol. Chem. 261, 285 (1986) | (6) K. Nichols, et al.; Neuroscience 51, 791 (1992) | (7) C. Libon, et al.; J. Leukoc. Biol. 53, 93 (1993) | (8) C.K. Mittal; Biochem. Biophys. Res. Commun. 193, 126 (1993) | (9) M. De la Fuente, et al.; Comp. Immunol. Microbiol. Infect. Dis. 16, 29 (1993) | (10) A. Pistelli, et al.; Biochem. Pharmacol. 47, 1737 (1994) | (11) A. Trinhle, et al.; BioTechniques 42|756 (2007) | (12) J.M. Ross; J. Visual. Exp. 57, e3266 (2011) | (13) Z.A. Siddiqi, et al.; Eur. J. Med. Chem. 57, 102 (2012)

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