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Chemodex
NBD-PZ
Product Details | |
---|---|
Synonyms | 4-Nitro-7-piperazino benzofurazan |
Product Type | Chemical |
Properties | |
Formula |
C10H11N5O3 |
MW | 249.23 |
CAS | 139332-66-4 |
Source/Host Chemicals | Synthetic. |
Purity Chemicals | ≥98% (NMR) |
Appearance | Red to brown powder. |
Solubility | Soluble in DMSO. |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | MVLWYDGJBGPXOL-UHFFFAOYSA-N |
Smiles | [O-][N+](=O)C1=CC=C(N2CCNCC2)C2=NON=C12 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet | Download PDF |
This common fluorescent reagent often used in carbohydrate analysis, is also useful in fluorescence visualization of lysosomes in live cell lines in culture. This fluorophore that has the unusual environmentally sensitive property of increasing fluorescence in low pH environments. Comparison of staining patterns with the common LysoTracker™ or LysoSensor™ dyes indicated comparable staining, albeit using a higher concentration of NBD-piperazine (1 μM versus 100 nM for the MP dyes) but at a lower cost per assay. Also used for the determination of mono- and diisocyantes in air samples, and determination of lactate, hydroxybutyrate and other intermediates in body fluids. The piperazino moiety reacts readily with isocyantes to form the corresponding urea-derivatives. The derivatives can be detected by LC with UV or fluorescent-detection. Spectral data: λex=470nm, λem=540nm.
(1) T.M. Toyo'oka, et al.; J. Chromatogr. 588, 61 (1991) | (2) T.M. Toyo'oka, et al.; Anal. Chim. Acta 465, 111 (2002) | (3) T. Santa, et al.; Biomed. Chromatogr. 16, 523 (2002) | (4) M. Vogel et al.; Anal. Chem. 74, 6418 (2002) | (5) A. Ostin et al.; J. Environ. Monit. 5, 100 (2003) | (6) J.-A. Lee et al.; Analytica Chimica Acta 534(2), 185 (2005) | (7) K. Ishiguro et al.; BioTechniques 45(4), 465 (2008)