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Chemodex
PBFI-AM

Product Details | |
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Synonyms | Potassium indicator PBFI-AM; Potassium-binding benzofuran isophthalate-AM; PBFI diacetate |
Product Type | Chemical |
Properties | |
Formula |
C58H62N2O24 |
MW | 1171.11 |
CAS | 124549-23-1 |
Source/Host Chemicals | Synthetic |
Purity Chemicals | ≥98% (HPCE) |
Appearance | Orange paste. |
Solubility | Soluble in DMSO or methanol. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | AUKRBWNSOWVABU-UHFFFAOYSA-N |
Smiles | O=C(OCOC(C)=O)C(C=C1)=CC(C(OCOC(C)=O)=O)=C1C2=CC3=CC(OC)=C(N4CCOCCOCCN(C5=C(OC)C=C6C(OC(C7=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C7)=C6)=C5)CCOCCOCC4)C=C3O2 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | -20°C |
Long Term Storage | -20°C |
Handling Advice |
Protect from light and moisture. Very light-sensitive. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
Product Specification Sheet | |
Datasheet |
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PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments. As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd=44 and 5.1mM with or without sodium, respectively). Spectral data: λEx=370nm; λEm=540nm in methanol.
(1) P. Jezek, et al.; J. Biol. Chem. 265, 10522 (1990) | (2) S. Lindberg; Planta 195, 525 (1995) | (3) C.D. Bortner, et al.; J. Biol. Chem. 272, 32436 (1997) | (4) S.J. Halperin & J.P. Lynch; J. Exp. Bot. 54, 2035 (2003) | (5) R.W. Sabnis; Handbook of biological dyes and stains (2010)