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Chemodex
Pico Green
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| Product Details | |
|---|---|
| Synonyms | Picogreen; 2-[Bis[3-(dimethylamino)propyl]amino]-4-[(3-methyl-2(3H)-benzothiazolylidene)methyl]-1-phenylquinolinium |
| Product Type | Chemical |
| Properties | |
| Formula | C34H42N5S |
| MW | 552.8 |
| CAS | 178918-98-4 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | Orange to red powder. |
| Solubility | Soluble in DMSO. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | ZYFVNVRFVHJEIU-UHFFFAOYSA-N |
| Smiles | CN(C)CCCN(C1=[N+](C2=CC=CC=C2)C3=C(C=CC=C3)C(/C=C4SC5=CC=CC=C5N/4C)=C1)CCCN(C)C |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
PicoGreen is a highly sensitive fluorescent dye specifically designed for the quantitation of double-stranded DNA (dsDNA) in solution. It exhibits exceptional selectivity, binding preferentially to dsDNA over single-stranded DNA, RNA, or free nucleotides, thereby minimizing background interference. Upon binding, PicoGreen produces a strong fluorescence signal with excitation/emission maxima around 480/520 nm, enabling detection of DNA at concentrations as low as picograms per milliliter. When PicoGreen binds to double-stranded nucleic acids, its fluorescence intensity increases approximately 1000-fold compared to unbound PicoGreen. This makes it an indispensable reagent in molecular biology for applications such as qPCR, DNA quantification in genomic and plasmid preparations, next-generation sequencing workflows, and high-throughput screening. Spectral Data: λex=480nm, λem=520nm.
Product References
(1) S.J. Ahn, et al.; Nucl. Acids Res. 24, 2625 (1996) | (2) V.L. Singer, et al.; Anal. Biochem. 249, 228 (1997) | (3) A.I. Dragan, et al.; Biophys. J. 99, 3010 (2010) | (4) E. Ban & A. Kim; Anal. Biochem. 692, 11557 (2024)