Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
Chemodex
Rhodamine B
Product Details | |
---|---|
Synonyms | Tetraetylrhodamine; Basic Violet 10; Brilliant Pink B; C.I. 45170; Rhodamine 610 chloride; Rheonine B; 9-(2-Carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride |
Product Type | Chemical |
Properties | |
Formula | C28H31ClN2O3 |
MW | 479.01 |
CAS | 81-88-9 |
RTECS | BP3675000 |
Source/Host Chemicals | Synthetic |
Purity Chemicals | ≥95% (HPLC) |
Appearance | Faint to dark green powder. |
Solubility | Soluble in water (10mg/ml), ethanol (20mg/ml) or DMSO. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | PYWVYCXTNDRMGF-UHFFFAOYSA-N |
Smiles | CCN(CC)C1=CC2=[O+]C(C=C(C=C3)N(CC)CC)=C3C(C(C=CC=C4)=C4C(O)=O)=C2C=C1.[Cl-] |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +20°C |
Long Term Storage | +20°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at RT. |
Documents | |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet | Download PDF |
Rhodamine B is a fluorescent xanthene dye widely used in various applications, including histology, due to its bright red to pink fluorescence when exposed to ultraviolet (UV) light. Rhodamine B is used as a tracer dye in water studies to track the movement of water, determine flow rates, and identify leaks in systems. It is commonly used in microscopy and flow cytometry for staining cells and tissues due to its ability to bind to cellular components and emit fluorescence. Rhodamine B is employed as a gain medium in dye lasers, where it helps in generating coherent light. Rhodamine B is tunable around 610nm when used as a laser dye. Rhodamine B has been incorporated in a FRET system based on lanthanide-doped nanoparticles for monitoring biological effects and has been used as a mitochondrial probe to study the energetic state of multi-drug resistant and sensitive cells. Rhodamine B is also a valuable tool in fluorescence microscopy and other fluorescence-based techniques. Spectral Data: λex=554nm, λem=627nm (EtOH). λex=546nm, λem=567nm (DMSO). Rhodamine B is used in the textile industry to dye fabrics, providing vibrant colors.
(1) M.F. Liisberg; Acta Anat. 69, 52 (1968) | (2) F.P. Clausen & E. Dabelsteen; Acta Pathol. Microbiol. Scand. 77, 169 (1969) | (3) J.R. Lakowicz, ete al.; J. Phys. Chem. 98, 334 (1994) | (4) F. Vult von Steyern, et al.; J. Histochem. Cytochem. 44, 267 (1996) | (5) V. Vondrejs & Z. Palkova; Methods Mol. Biol. 53, 319 (1996) | (6) P. Reungpatthanaphong, et al.; J. Biochem. Biophys. Methods 57, 1 (2003) | (7) W. Di, et al.; Nanotechnol. 21, 455703 (2010) | (8) Y. Ding, et al.; Chem. Commun. 49, 7797 (2013) | (9) C. Xie, et al.; Eur. J. Pharm. Biopharm. 85, 541 (2013) | (10) L. Dong, et al.; J. Nanosci. Nanotechnol. 18, 2441 (2018) | (11) G. Bao, et al.; Chempluschem. 84, 816 (2019) | (12) S. Hoenke, et al.; Molecules 25, 5443 (2020) | (13) L.M. Skjolding, et al.; Water Res. 197, 117109 (2021) | (14) A.K. Al-Buriahi, et al.; Chemosphere 287, 132162 (2022) (Review) | (15) F. Deng, et al.; Spectrochim. Acta A Mol. Biomol. Spectrosc. 268, 120662 (2022)