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Chemodex
Resorufin-β-D-galactopyranoside
90
CHF
CHF 90.00
In stock
CDX-R0005-M01010 mgCHF 90.00
| Product Details | |
|---|---|
| Synonyms | 3-Phenoxazone 7-(β-D-galactopyranoside) |
| Product Type | Chemical |
| Properties | |
| Formula |
C18H17NO8 |
| MW | 375.33 |
| CAS | 95079-19-9 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥97% (TLC) |
| Appearance | Orange to brown solid. |
| Solubility | Soluble in DMSO (20mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | QULZFZMEBOATFS-DISONHOPSA-N |
| Smiles | O=C1C=C2OC3=CC(O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)=CC=C3N=C2C=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Resorufin β-D-galactopyranoside is a fluorogenic substrate for β-galactosidase. Upon enzymatic cleavage by the enzymes, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: λex=571nm, λem=584nm. It is used in several applications, specifically in compound screens, where it is advantageous because it is less prone to compound autofluorescence, and thus produces less false positives. It is widely used as substrate in high throughput screening of galactosidase inhibitors.
Product References
(1) J. Hofmann & M. Sernetz; Anal. Chim. Acta 163, 67 (1984) | (2) K.D. Wittrup & J.E. Bailey; Cytometry 9, 394 (1988) | (3) A.G. Hadd, et al.; Anal. Chem. 69, 3407 (1997) | (4) M.J. Eggertson & D.B. Craig; Biomed. Chromatogr. 13, 516 (1999) | (5) G.K. Shoemaker, et al.; Biochemistry 42, 1707 (2003) | (6) S. Jambovane, et al.; Anal. Chem. 81, 3239 (2009)