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Chemodex
TMB
41
CHF
CHF 41.00
In stock
CDX-T0002-G0011 gCHF 41.00
CDX-T0002-G0055 gCHF 102.00
CDX-T0002-G02525 gCHF 339.00
| Product Details | |
|---|---|
| Synonyms | 3,3',5,5'-Tetramethylbenzidine; BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; TMBZ |
| Product Type | Chemical |
| Properties | |
| Formula |
C16H20N2 |
| MW | 240.34 |
| CAS | 54827-17-7 |
| RTECS | DV2300000 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥99% (HPLC) |
| Appearance | White to light yellow powder. |
| Solubility | Soluble in DMSO. Insoluble in water. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | UAIUNKRWKOVEES-UHFFFAOYSA-N |
| Smiles | CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
3,3‘,5,5‘-Tetramethylbenzidine (TMB) chromogenic peroxidase sensitive dye. TMB is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm. A sensitive and specific reagent for the detection of blood, assay of haemoglobin and assay of horseradish peroxidase (HRP).
Product References
(1) R.C. Lijana & M.C. Williams; J. Lab. Clin. Med. 94, 266 (1979) | (2) E.S. Bos, et al.; J. Immunoassay 2, 187 (1981) | (3) D.B. Rye, et al.; J. Histochem. Cytochem. 32, 1145 (1984) | (4) A.K. Goka & M.J. Farthing; J. Immunoassay 8, 29 (1987) | (5) J.L. McKimm-Breschkin; J. Immunol. Methods 135, 277 (1990) | (6) A. Frey, et al.; J. Immunol. Methods 233, 47 (2000) | (7) R. Georgescu; Methods Mol. Biol. 230, 171 (2003) | (8) L. Zhang & J. Du; Spectrochim. Acta A Mol. Biomol. Spectrosc. 158, 24 (2016) | (9) C. Lei, et al.; Talanta 175, 457 (2017)