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Chemodex
Tetramethylrhodamine ethyl ester perchlorate
CHF 0.00
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| Product Details | |
|---|---|
| Synonyms | TMRE; TMR ethyl ester; T 669 |
| Product Type | Chemical |
| Properties | |
| Formula | C26H27N2O3 . ClO4 |
| MW | 514.95 |
| CAS | 115532-52-0 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥90% (HPCE) |
| Appearance | Green to brown to purple powder. |
| Solubility | Soluble in DMSO (30mg/ml) or DMF (30mg/ml). Slightly soluble in methanol or water. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | NBAOBNBFGNQAEJ-UHFFFAOYSA-M |
| Smiles | CN(C)C1=CC=C2C(OC(C3=C2C4=C(C(OCC)=O)C=CC=C4)=C/C(C=C3)=[N+](C)/C)=C1.O=Cl(=O)([O-])=O |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +20°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Tetramethylrhodamine ethyl ester perchlorate (TMRE) is a cell-permeant, cationic fluorescent dye widely used to assess mitochondrial membrane potential and for labeling mitochondria in live-cell imaging and flow cytometry. By selectively accumulating in active mitochondria according to their membrane potential, TMRE enables real-time monitoring of mitochondrial function, bioenergetics, and apoptosis-related depolarization events. Due to the polarization of the mitochondrial membrane, TMRE is taken up into healthy mitochondria, but when the membrane is depolarized, it is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability. TMRE is commonly applied in studies of neurobiology, cardiology, toxicology, and metabolic research to investigate mitochondrial dynamics, oxidative stress, and cellular responses to pharmacological agents. TMRE is slightly more hydrophobic than TMRM. Spectral Data: λEx=555 nm; λEx=574 nm (red-orange).
Product References
(1) D.L. Farkas, et al.; Biophys. J. 56, 1053 (1989) | (2) R.C. Scaduto & L.W. Grotyohann; Biophys. J. 76, 469 (1999) | (3) K. Trudeau, et al.; Invest. Ophthalmol. Vis. Sci. 52, 8657 (2011) | (4) N.S. Barteneva, et al.; J. Histochem. Cytochem. 62, 265 (2014) | (5) L.C. Crowley, et al.; Cold Spring Harb. Protoc. 2016(12) (2016)