Chemodex

Tetrazolium Blue Chloride

Name: Tetrazolium Blue Chloride
Brand: Chemodex
SKU: CDX-T0136
Price: 39.00 CHF
Availability: InStock

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CDX-T0136-G0011 gCHF 39.00

CDX-T0136-G0055 gCHF 116.00

CDX-T0136-G02525 gCHF 464.00

Specifications / Handling

More Information
Product Details
Synonyms	BTC; Blue Tetrazolium chloride; TZ; NSC 27623; Ditetrazolium chloride; 3,3'-Dianisole-4,4'-bis(3,5-diphenyltetrazolium chloride)
Product Type	Chemical
Properties
Formula	C₄₀H₃₂N₈O₂ . 2Cl
MW	727.65
CAS	1871-22-3
Source/Host Chemicals	Synthetic
Purity Chemicals	≥97% (HPLC)
Appearance	Yellow crystalline solid.
Solubility	Soluble in methanol (50mg/ml).
Identity	Determined by ¹H-NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	MUUHXGOJWVMBDY-UHFFFAOYSA-L
Smiles	COC1=CC(C2=CC(OC)=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=CC=C5)C=C2)=CC=C1N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-]
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

Tetrazolium Blue Chloride (Nitro Blue Tetrazolium, NBT) is a redox-sensitive dye widely used as a chromogenic reagent for detecting enzymatic activity, reactive oxygen species (ROS), and cellular respiration. Upon reduction by dehydrogenases or superoxide radicals, it forms an insoluble blue formazan precipitate, enabling visual or spectrophotometric quantification of metabolic activity. NBT is commonly used as a colorimetric reagent and applied in microbiology, histochemistry, and plant and animal cell research to assess oxidative burst, mitochondrial function, and electron transport chain activity. Spectral Data: λabs ~540 nm, with a wide wavelength range of 480-600 nm.

Product-specific References

(1) V.A. DeBari &M.A. Needle; J. Immunol. Meth. 40, 89 (1981) | (2) C.K. Jue & P.N. Lipke; J. Biochem. Biophys. Meth. 11, 109 (1985) | (3) M.V. Berridge, et al.; Biotechnol. Annu. Rev. 11, 127 (2005) | (4) L.J. Yan, et al.; Electrophoresis 28, 1036 (2007) | (5) C. Pannecouque, et al.; Nat. Protoc. 3, 427 (2008) | (6) R.W. Sabnis; Handbook of biological dyes and stains (2010)

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