Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
Chemodex
Tetrazolium Blue Chloride
CHF 0.00
Out of stock
NEW
| Product Details | |
|---|---|
| Synonyms | BTC; Blue Tetrazolium chloride; TZ; NSC 27623; Ditetrazolium chloride; 3,3'-Dianisole-4,4'-bis(3,5-diphenyltetrazolium chloride) |
| Product Type | Chemical |
| Properties | |
| Formula | C40H32N8O2 . 2Cl |
| MW | 727.65 |
| CAS | 1871-22-3 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥97% (HPLC) |
| Appearance | Yellow crystalline solid. |
| Solubility | Soluble in methanol (50mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | MUUHXGOJWVMBDY-UHFFFAOYSA-L |
| Smiles | COC1=CC(C2=CC(OC)=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=CC=C5)C=C2)=CC=C1N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-] |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Tetrazolium Blue Chloride (Nitro Blue Tetrazolium, NBT) is a redox-sensitive dye widely used as a chromogenic reagent for detecting enzymatic activity, reactive oxygen species (ROS), and cellular respiration. Upon reduction by dehydrogenases or superoxide radicals, it forms an insoluble blue formazan precipitate, enabling visual or spectrophotometric quantification of metabolic activity. NBT is commonly used as a colorimetric reagent and applied in microbiology, histochemistry, and plant and animal cell research to assess oxidative burst, mitochondrial function, and electron transport chain activity. Spectral Data: λabs ~540 nm, with a wide wavelength range of 480-600 nm.
Product References
(1) V.A. DeBari &M.A. Needle; J. Immunol. Meth. 40, 89 (1981) | (2) C.K. Jue & P.N. Lipke; J. Biochem. Biophys. Meth. 11, 109 (1985) | (3) M.V. Berridge, et al.; Biotechnol. Annu. Rev. 11, 127 (2005) | (4) L.J. Yan, et al.; Electrophoresis 28, 1036 (2007) | (5) C. Pannecouque, et al.; Nat. Protoc. 3, 427 (2008) | (6) R.W. Sabnis; Handbook of biological dyes and stains (2010)