ZnAF-2F DA Solution
|Purity Chemicals||≥95% (HPCE)|
|Solubility||Soluble in DMSO.|
|Concentration||5mM in DMSO (1mg in 0.29ml DMSO)|
|Identity||Determined by 1H-NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Keep cool and dry.
Protect from light.
|Use/Stability||Stable for at least 2 years after receipt when stored at -20°C.|
|Product Specification Sheet|
For application to living cells, the diacetyl derivative of ZnAF-2F, ZnAF-2F DA, was synthesized. ZnAF-2F DA can permeate through the cell membrane, and is hydrolyzed by esterase in the cytosol to yield ZnAF-2F, which is retained in the cells. Using ZnAF-2F DA we could measure the changes of intracellular Zn2+ in cultured cells and hippocampal slices. Spectral data: λex 492nm; λem 515nm in PBS.
(1) S. Ueno, et al.; J. Cell Biol. 158, 215 (2002) | (2) T. Hirano, et al.; JACS 124, 6555 (2002)