FLT3 Ligand (human) (rec.) (His)

CHF 240.00
In stock
CHI-HF-201FLT3L-C01010 µgCHF 240.00
CHI-HF-201FLT3L-C05050 µgCHF 710.00
More Information
Product Details
Synonyms FLT3L; FMS-like Tyrosine Kinase 3 Ligand
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Human FLT3L (aa 27 -184) is fused at the C-terminus to a His-tag.

Crossreactivity Human
Mouse
Biological Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

MW ~25kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.001EU/μg purified protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Other Product Data

NCBI reference AAI26294.1: FLT3L (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

FMS-like tyrosine kinase 3 ligand (FLT3L) acts as a growth factor that increases the number of immune cells (lymphocytes (B cells and T cells)) by activating the hematopoietic progenitors. It binds to FLT3 (CD135) which is found on multipotent progenitor (MPP) and common lymphoid progenitor (CLP) cells in mice. FLT3L induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3L is crucial for steady-state pDC (plasmacytoid dendritic cells) and cDC (classical dendritic cells) development. Deficiency of FLT3L causes a dramatic decrease in DC numbers, whereas increasing its availability increase in DC numbers.

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