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AdipoGen Life Sciences
Fc (human):FLT3 Ligand (human) (rec.)
240
CHF
CHF 240.00
In stock
AG-40B-0119-C01010 µgCHF 240.00
AG-40B-0119-30103 x 10 µgCHF 480.00
AG-40B-0119-C100100 µgCHF 960.00
Figure 1: Influence of FLT3L treatment on lymphocyte subpopulations. Method: C57BL/6 mice were injected intraperitoneally with 10 μg daily or were left untreated. (A) Percentages of indicated splenic subpopulations in FLT3L KO, WT control or WT-treated mice. (B) Absolute cell numbers of indicated splenic subpopulations in FLT3L KO, WT control, or WT-treated mice.
| Product Details | |
|---|---|
| Synonyms | FMS-like Tyrosine Kinase 3 Ligand |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
The receptor-binding domain of human FLT3L (aa27-185) is fused at the N-terminus to the Fc portion of human IgG1. |
| Crossreactivity |
Human Mouse |
| Specificity |
Binds to human and mouse FLT3 receptor. |
| Biological Activity |
In vitro and in vivo generation of conventional and plasmacytoid dendritic cells (DC). FLT3L increases in vivo both NK and DC cells numbers in the spleen when mice are injected intraperitoneally with 2 or 10 µg, daily for 10 days (slightly higher effect at 10 µg). |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A affinity purified. |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS (sterile). |
| Protein Negative Control | |
| Other Product Data |
UniProt link P49771: FLT3L (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
FMS-like tyrosine kinase 3 ligand (FLT3L) acts as a growth factor that increases the number of immune cells (lymphocytes (B cells and T cells)) by activating the hematopoietic progenitors. It binds to FLT3 (CD135) which is found on multipotent progenitor (MPP) and common lymphoid progenitor (CLP) cells in mice. FLT3L induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3L is crucial for steady-state pDC (plasmacytoid dendritic cells) and cDC (classical dendritic cells) development. Deficiency of FLT3L causes a dramatic decrease in DC numbers, whereas increasing its availability increase in DC numbers.
Product References
- Expansion of peripheral naturally occurring T regulatory cells by Fms-like tyrosine kinase 3 ligand treatment: L.K. Swee, et al.; Blood 113, 6277 (2009)
- In vivo evidence for an instructive role of fms-like tyrosine kinase-3 (FLT3) ligand in hematopoietic development: P. Tsapogas, et al.; Haematologica 99, 638 (2014)
- Flt3 Ligand Regulates the Development of Innate Lymphoid Cells in Fetal and Adult Mice: A. Baerenwaldt, et al.; J. Immunol. 196, 2561 (2016)
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