Tim-4 (human):Fc (human) (rec.)
|Synonyms||TIM4; TIMD4; T Cell Immunoglobulin and Mucin Domain-containing Protein 4; T Cell Membrane Protein 4|
The extracellular domain of human Tim-4 (aa 25-315) is fused to the N-terminus of the Fc region of human IgG1.
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
|Endotoxin Content||<0.06EU/μg protein (LAL test; Lonza).|
|Reconstitution||Reconstitute with 100 µl sterile water. Add 1X PBS to the desired protein concentration.|
|Formulation||Lyophilized from 0.2μm-filtered solution in PBS.|
|Protein Negative Control|
|Other Product Data||
NCBI reference NP_612388.2: Tim-4 (human)
|Declaration||Manufactured by Chimerigen.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||Avoid freeze/thaw cycles.|
Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. TIM-4 (T cell; immunoglobulin; Mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIM-4 is exclusively expressed on antigen-presenting cells, where it mediates phagocytosis of apoptotic cells and plays an important role in maintaining tolerance. TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma. Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells. The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells.
- TIM-4 is the ligand for TIM-1, and the TIM-1–TIM-4 interaction regulates T cell proliferation: J. Hartt Meyers, et al.; J. Immunol. 6, 455 (2005)
- Tim-4 expressed on antigen-presenting cells induces T cell expansion and survival: R. Rodriguez-Manzanet, et al.; J. Immunol. 180, 4706 (2008)